The Online Regional Training Course on Sampling Gear Design for Onboard Fisheries Resource Survey was organized by the SEAFDEC Training Department (SEAFDEC/TD) from 31 August to 4 September 2020 at SEAFDEC/TD office via Google Meet platform. This Online Training is a part of SEAFDEC’s initiatives to support the Member Countries for developing the capacities of national human resources to conduct a fisheries resource survey. There were nineteen (19) participants (7 females and 12 males) who attended this activity. The Online Training focused on the introduction and problem of fisheries resource survey, sampling gear selection, fishing gear design, sampling process and data recording, CPUE standardization, and fisheries resource survey design. The Online Training was designed to achieve improved knowledge and gained experience of researchers and fisheries officers of the Member Countries to design sampling gear and plan sampling sites through fisheries resource survey researcher network in the Southeast Asian region.