The SEAFDEC Training Department (SEAFDEC/TD) organized “Online Regional Training Course on Implementation of Port State Measures for Inspection” from 26 to 29 October 2021 in collaboration with partners, namely the Food and Agriculture Organization of the United Nations (FAO), the National Oceanic & Atmospheric Administration (NOAA), and the Department of Fisheries of Thailand (DOF Thailand). The Training aims to 1) update the status and the implementation of the PSMA, 2) build up the understanding and capacity of the participants on the PSM, and 3) enhance knowledge, skills, and experience of the participants on inspection to support the PSM and port control implementation. There were 44 participants (6 female and 38 male) from AMSs who have been engaged in the implementation of the PSM and/or port control activities at the inspection level. Moreover, it is also expected that the participants should have potential to serve as trainer for PSM inspection in their respective country in the future attended in this training. The expected outputs from this Training included understanding on the status and the implementation of the PSMA; increasing knowledge, skills, and experience of participants on inspection to support Port State Measures and port control implementation; and further strengthening of regional cooperation to support the implementation of PSM in Southeast Asia.