The SEAFDEC Training Department (SEAFDEC/TD) organized “The Regional Training Course on Fish Larvae Phase I: Larval Fish Identification and Fish Early Life History Science” from 16 to 27 November 2022, at SEAFDEC/TD, Samut Prakan, Thailand. The main aim is to improve the knowledge and skills on fish early life history science and identify the larval fish by lecture and practice. There were 26 participants who are fisheries biologists from ASEAN Member States (AMSs) attended this training. The expertise in larval fish identification from the Seikai National Fisheries Research Institute, Japan; the Department of Fisheries, Thailand; Kasetsart University, Thailand; University of the Ryukyus, Japan; and University of Nottingham, Malaysia was invited to be resource persons. In the end, the participants understood and improved their knowledge and skill in larval fish identification for managing the fish stock at national and sub-regional levels through strengthened regional cooperation on larval fish networking. This training was organized in collaboration under the project of 1) Establishment and Operation of a Regional System of Fisheries Refugia in the South China Sea and the Gulf of Thailand, and 2) Sustainable Utilization of Fisheries Resources and Resources Enhancement in Southeast Asia.